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Table 1 Primer and probe sequences used for qRT-PCR

From: CSF1R antagonism limits local restimulation of antiviral CD8+ T cells during viral encephalitis

Target Primer sequence (5′➔3′)
GAPDH
 Fwd GGC AAA TTC AAC GGC ACA GT
 Rev AGA TGG TGA TGG GCT TCC C
IFNα
 Fwd CTT CCA CAG GAT CAC TGT GTA CCT
 Rev TTC TGC TCT GAC CAC CTC CC
IFNβ
 Fwd CTG GAG CAG CTG AAT GGA AAG
 Rev CTT CTC CGT CAT CTC CAT AGG G
IFNγ
 Fwd AAC GCT ACA CAC TGC ATC TTG G
 Rev GCC GTG GCA GTA ACA GCC
TNFα
 Fwd GCA CAG AAA GCA TGA TCC G
 Rev GCC CCC CAT CTT TTG GG
iNOS
 Fwd GGA GCC TTT AGA CCT CAA CAG A
 Rev TGA ACG AGG AGG GTG GTG
CD86
 Fwd ACG ATG GAC CCC AGA TGC ACC A
 Rev GCG TCT CCA CGG AAA CAG CA
CD68
 Fwd CCA CAG GCA GCA CAG TGG ACA
 Rev TCC ACA GCA GAA GCT TTG GCC C
Arg1
 Fwd TTA GGC CAA GGT GCT TGC TGC C
 Rev TAC CAT GGC CCT GAG GAG GTT C
TGFβ
 Fwd TAC TAT GCT AAA GAG GTC ACC C
 Rev CTT CCC GAA TGT CTG ACG TAT TG
IL10
 Fwd TTG GAA TTC CCT GGG TGA GAA
 Rev GGA GAA ATC GAT GAC AGC GC
WNV
 Fwd TCA GCG ATC TCT CCA CCA AAG
 Rev GGG TCA GCA CGT TTG TCA TTG
 Probe /56-FAM/TGC CCG ACC /ZEN/ ATG GGA GAA GCT C/3IABkFQ/