Fig. 3

The effects of differential expression of CatC on microglia and proinflammatory cytokines in the brain. After spatial probe test, the activation of microglia was detected by Iba1 IHC staining in cortex and hippocampus of untreated mice (A) and LPS (i.p.)-injected mice (D). The levels of TNF-α and IL-1β were measured in untreated mice (B, C) and LPS-injected mice (E, F). The levels of TNF-α and IL-1β were determined by qRT-PCR and ELISA. Scale bars, 100 μm (main panels), 25 μm (insets). *P ≤ 0.05, **P ≤ 0.01. n = 3 (A–D), n = 3–5 (E, F)