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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Cathepsin C promotes microglia M1 polarization and aggravates neuroinflammation via activation of Ca2+-dependent PKC/p38MAPK/NF-κB pathway

Fig. 8

CatC stimulation increased the concentration of intracellular Ca2+ and induced PKC activation. BV2 cells were stimulated with active CatC (100 ng/ml) and co-stimulated with E-64 (50 μM) or pre-incubated with MK-801 (10 μM) for 6 h. Then, intracellular Ca2+ concentration was measured by flow cytometry (A). Primary cultured microglia form WT mice and BV2 cells were stimulated with active CatC (100 ng/ml), or co-stimulated with E-64 (50 μM), or treated with MK-801 (10 μM) prior to CatC stimulation for 12 h. The expressions of p-PKC and t-PKC were quantified by western blot analysis in primary cultured microglia (B) and BV2 cells (C). The bands shown are representative of three independent experiments. GAPDH as a loading control. The statistics are shown as increased folds of treatment groups to the control. The data represent mean ± SEM, n = 3. *P < 0.05, **P < 0.01

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