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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: Cathepsin C promotes microglia M1 polarization and aggravates neuroinflammation via activation of Ca2+-dependent PKC/p38MAPK/NF-κB pathway

Fig. 9

CatC activated p38 MAPK/NF-κB signaling pathway in microglia. A Primary cultured microglia from WT mice were treated with active CatC (100 ng/ml), or co-stimulated with E-64 (50 μM), or pretreated with MK-801 (10 μM). The expressions of p-p38, t-p38, p-IκBα, t-IκBα, p-p65, and t-p65 were quantified by western blot analysis. The levels of p-p38/t-p38 (a), p-IκBα/t-IκBα (b), p-p65/t-p65 (c) were shown as increased folds of treatment groups relative to control group. B Activation of p38 MAPK/NF-κB signaling pathway was examined in BV2 cells. The bands shown are representative of three independent experiments. The data represent mean ± SEM, n = 3. *P < 0.05, **P < 0.01

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