Fig. 4

Effects of Apelin 13 on the cytotoxicity and apoptosis induced by I/R in PC12 cells. a PC12 cells viability is assessed by measuring the CCK8 reduction. Results were shown as fold of control. b Effects of Apelin 13 on the levels of LDH in PC12 cell subjected to I/R. c Effects of Apelin 13 on PC12 cells apoptosis induced by I/R. PC12 cells were subjected to I/R with or without Apelin 13 pretreatment, then double stained with Annexin V/propidium iodide (PI). d Effects of Apelin 13 on the expression levels of Bax, Bcl-2, cleaved PARP, cleaved caspase-3, and cleaved caspase-9 in PC12 cell subjected to I/R. e ROS level is measured by a DHE kit using a laser confocal microscope. The effects of Apelin 13 on the levels of MDA (f), CAT (g), SOD (h), 8-Oxo-dG (i), IL-1(j), TNF-α(k), and COX-2 (l) were measured as described in the “Methods” section. The columns and error bars were represented as means ± SD. ^##P < 0.01 vs. the control group; *P < 0.05, **P < 0.01 vs. the I/R treatment group