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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Neuroprotective effect of Apelin 13 on ischemic stroke by activating AMPK/GSK-3β/Nrf2 signaling

Fig. 5

Effects of Apelin 13 on expression of Nrf2-related antioxidant enzymes in PC12 cells subjected to I/R. a PC12 cells were pretreated with Apelin 13 for 6 h, then subjected to I/R. The expression levels of Nrf2, NQO-1, and HO-1 in cytoplasm were measured by western bolting as the method motioned in the “Methods” section part. b The expression level of Nrf2 in nuclear is measured using the nuclear extract. c Effects of Apelin 13 on the activity of ARE determined by ARE luciferase report assay. d The cells were treated with Nrf2-specific siRNA (40 nM) or scrb siRNA for 48 h and Nrf2 expression level in cytoplasm was measured by western bolting. e Effects of siNrf2 on the protein expression levels of NQO-1, HO-1, and SOD. TNF-α (f), ROS (g), apoptosis rate (h), and cell viability (i) were measured after different treatments. The columns and error bars were represented as means ± SD. ##P < 0.01 vs. the control group; **P < 0.01 vs. the I/R treatment group. &&P < 0.05 vs. the scrb control RNA group

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