Fig. 4

Monocytes, macrophages and microglia subpopulation analysis in the brain prior to and after GBM implantation. a Following the cytometry gating used in Fig. 2d, the remaining myeloid subpopulations of monocytes, macrophages (Mɸ) and microglia were further separated using Ly6C/MHCII/MerTK/CD64 into monocytes, undifferentiated Mɸ and a combination of differentiated Mɸ and microglia. The combined group of differentiated Mɸ and microglia were separated using CD11b/CD45. All brain samples were analysed using flow cytometry (n = 3/group). b Bar graph showing the relative glioma-associated macrophages and microglia (GAMM) subpopulations in tumour-bearing hemisphere of GBM-implanted mice. All samples were analysed using a two-way ANOVA with Tukey’s post hoc correction for multiple comparisons. Error bar represents the SEM. **** or ^####p < 0.0001; ***or ^###p < 0.001; **or ^##p < 0.01. One mouse from the NMC group was excluded due to reflux of tumour cells during surgery, thus providing unreliable downstream analysis