Fig. 6

AMPK inhibitor Compound C reverses the effects of AICAR. Compound C (20 μg, 80 μg/20 μl) was administrated at post-injection of CFA day 4 before AICAR (15 μg/20 μl) treatment 30 min. Skin tissues were collected at 2 h after AICAR treatment. Representative gels and quantification for pro-IL-1β and IL-1β p17 bands are shown (a, b). Data are expressed as mean ± SEM. One-way ANOVA revealed a significant difference at ^*P < 0.05 vs. Control group and ^#P < 0.05 vs. CFA+VEH group (n = 4 mice/group). The abbreviations used here are VEH (vehicle of AICAR), AI (AICAR) and CC (Compound C). c Immunostaining of skin sections showing the co-localization of CX3CR1-GFP ⁺ cells and p-AMPK in inflamed skins on the fourth day (c). Scale bar represents 50 μm