Fig. 3
Ac2-26 does not reduce the microglia recruitment induced by SE. a Resting microglia from control groups (Naive and Sham) characterised by a fully ramified form (red arrows). SE and SE+Ac2-26 groups presented bushy and ameboid cells with few and short cytoplasmic prolongations (white arrows). Counterstain, Haematoxylin. Bars, 50 μm. b Quantification of Iba-1+cells in the in CA1 (anterior and posterior) and CA3 regions, right and left sides. Dot plot graph of Iba-1+ cells/mm2 (n = 6 animals/group). ###P < 0.001 vs. Naive; ***P < 0.001 vs. SHAM (Kruskal-Wallis, Dunn post-test). c Western blot analysis to measure Iba-1 (~ 17 kDa) in the pooled extracts of rat hippocampus (n = 3 animals/group) from all experimental groups. GAPDH was used as a protein loading control. Immunoreactive bands for Iba-1 were semi-quantified by densitometry and are expressed as arbitrary units (a.u.) of the ratio of Iba-1/GAPDH (data represent one illustrative blot from four independent experiments; mean ± SEM)