Fig. 6
Ac2-26 decreased Fpr2 and ERK levels in hippocampal neurons. a, b SE group showed hippocampal neurons with intense immunostain for Fpr2 and phosphorylated ERK (arrows) compared to the SHAM and SE+Ac2-26 groups. No immunostaining was detected in the sample used as a negative control. Counterstain, haematoxylin. Bars, 20 μm. c Densitometric analysis of Fpr2 expression in the hippocampal neurons showed increased levels of this receptor, abrogated by Ac2-26 treatment. Data represent mean ± SEM of Fpr2 expression (a.u.; n = 5 animals/group). ##P < 0.01, ##P < 0.001 vs. Naive; ***P < 0.001 vs. Sham; +++P < 0.05 vs. SE (Kruskal-Wallis, Dunn post-test). d, e: Immunoassays for Fpr2 (~ 40 kDa) and pERK (~ 42–44 kDa) detection in the pooled extracts of rat hippocampus (n = 3 animals/group) from all experimental groups. GAPDH and total ERK were used as protein loading controls. Immunoreactive bands for proteins were semi-quantified by densitometry and are expressed as arbitrary units (a.u.) of the ratio of Fpr2/GAPDH or pERK/ERK (data represent one illustrative blot from four independent experiments). #P < 0.05 vs. Naive, +P < 0.05 vs. SE (ANOVA, Bonferroni post-test)