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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Oligodendrocyte-specific ATF4 inactivation does not influence the development of EAE

Fig. 2

ATF4 inactivation specifically in oligodendrocytes did not affect the viability and function of oligodendrocytes under normal conditions. a PCR analysis showed that the floxed ATF4 alleles were presented in the brain (1), spinal cord (2), optic nerve (3), sciatic nerve (4), heart (5), liver (6), spleen (7), lung (8), and kidney (9) of ATF4 cKO mice and control mice; however, ATF4 knockout (KO) alleles were only presented in the brain (1), spinal cord (2), optic nerve (3), and sciatic nerve (4) of ATF4 cKO mice. N = 3 animals. b, c Western blot analysis showed that the protein level of ATF4 was significantly decreased in the brain of 10-week-old ATF4 cKO mice compared to control mice. N = 3 animals. df CC1 IHC showed that oligodendrocyte-specific ATF4 inactivation did not significantly change oligodendrocyte numbers in the spinal cord (SC), corpus callosum (CC, representative images not shown), or cerebellum (CB, representative images not shown) of 10-week-old mice. N = 4 animals. gj MBP IHC showed that oligodendrocyte-specific ATF4 inactivation did not significantly affect the degree of myelination in the spinal cord (g, h) or corpus callosum (i, j) of 10-week-old mice. N = 4 animals. Scale bars: d, e, 50 μm; gj, 100 μm. Error bars represent SD, *P < 0.05. n.s. not significant

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