Fig. 4

ATF4 inactivation specifically in oligodendrocytes did not alter EAE disease severity. a Mean clinical score. Error bars represent SEM, N = 9 animals. b, c Western blots analysis showed that the levels of ATF4 and CHOP were significantly increased in the brain of control mice with EAE at PID 21 compared to naïve control mice. Moreover, the levels of ATF4 and CHOP were significantly decreased in the brain of ATF4 cKO mice with EAE at PID 21 compared to control mice with EAE. N = 3 animals. d ASPA and CHOP double immunostaining showed that CHOP was detectable in the majority of ASPA positive oligodendrocytes (arrows) in the spinal cord of control mice with EAE at PID21, but was undetectable in ASPA-positive oligodendrocytes of ATF4 cKO mice with EAE. Scale bars: d, 20 μm. N = 4 animals. Error bars represent SD, *P < 0.05, **P < 0.01. n.s. not significant