Fig. 5

ATF4 inactivation specifically in oligodendrocytes did not alter oligodendrocyte loss, demyelination, or axon degeneration in the CNS of EAE mice. a–c ASPA immunostaining showed that ATF4 inactivation specifically in oligodendrocytes did not significantly change the degree of reduction of oligodendrocyte numbers in the lumbar spinal cord of EAE mice at PID 21. d–f MBP IHC showed that ATF4 inactivation specifically in oligodendrocytes did not significantly change the degree of demyelination in the lumbar spinal cord of EAE mice at PID 21. g–i SMI 31 immunostaining revealed that the number of total axons in the demyelinated lesions in the lumbar spinal cord of ATF4 cKO mice was comparable to control mice at PID21. j–l SMI 32 immunostaining showed that ATF4 inactivation in oligodendrocytes did not significantly change the number of degenerating axons in the demyelinated lesion in the lumbar spinal cord of EAE mice at PID 21. Scale bars: a, b, g, h, j, k, 20 μm; d, e, 100 μm; N = 4 animals. Error bars represent SD, n.s. not significant