Skip to main content

Advertisement

Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Oligodendrocyte-specific ATF4 inactivation does not influence the development of EAE

Fig. 8

ATF4 inactivation specifically in oligodendrocytes did not change the infiltration of inflammatory cells in the CNS gray matter during EAE. a, b, g CD3 immunostaining showed that ATF4 inactivation specifically in oligodendrocytes did not significantly change the number of CD3-positive T cells in the anterior horn of the spinal cord (SC) in EAE mice at PID21. Red fluorescence: CD3, blue fluorescence: DAPI. Arrows pointed out representative CD3-positive T cells. c, d, g CD3 immunostaining showed that ATF4 inactivation specifically in oligodendrocytes did not significantly change the number of CD3 positive T cells in the layer V of the primary motor cortex (MC) in EAE mice at PID21. Red fluorescence: CD3, blue fluorescence: DAPI. Arrows pointed out representative CD3 positive T cells. eg CD3 immunostaining showed that ATF4 inactivation specifically in oligodendrocytes did not significantly change the number of CD3 positive T cells in the molecular layer and internal granular layer of the cerebellum (CB) in EAE mice at PID21. Red fluorescence: CD3, blue fluorescence: DAPI. Arrows pointed out representative CD3-positive T cells. h, i, n CD11b immunostaining showed that ATF4 inactivation specifically in oligodendrocytes did not significantly change the number of CD11b-positive microglia/macrophages in the anterior horn of the spinal cord (SC) in EAE mice at PID21. Red fluorescence: CD11b, blue fluorescence: DAPI. Arrows pointed out representative CD11b-positive microglia/macrophages. j, k, n CD11b immunostaining showed that ATF4 inactivation specifically in oligodendrocytes did not significantly change the number of CD11b-positive microglia/macrophages in the layer V of the primary motor cortex (MC) in EAE mice at PID21. Red fluorescence: CD11b, blue fluorescence: DAPI. Arrows pointed out representative CD11b-positive microglia/macrophages. ln CD11b immunostaining showed that ATF4 inactivation specifically in oligodendrocytes did not significantly change the number of CD11b-positive microglia/macrophages in the molecular layer and internal granular layer of the cerebellum (CB) in EAE mice at PID21. Red fluorescence: CD11b, blue fluorescence: DAPI. Arrows pointed out representative CD11b-positive microglia/macrophages. Scale bars: a–f, hm, 50 μm. N = 4 animals. Error bars represent SD, n.s. not significant

Back to article page