Fig. 8
From: Oligodendrocyte-specific ATF4 inactivation does not influence the development of EAE
ATF4 inactivation specifically in oligodendrocytes did not change the infiltration of inflammatory cells in the CNS gray matter during EAE. a, b, g CD3 immunostaining showed that ATF4 inactivation specifically in oligodendrocytes did not significantly change the number of CD3-positive T cells in the anterior horn of the spinal cord (SC) in EAE mice at PID21. Red fluorescence: CD3, blue fluorescence: DAPI. Arrows pointed out representative CD3-positive T cells. c, d, g CD3 immunostaining showed that ATF4 inactivation specifically in oligodendrocytes did not significantly change the number of CD3 positive T cells in the layer V of the primary motor cortex (MC) in EAE mice at PID21. Red fluorescence: CD3, blue fluorescence: DAPI. Arrows pointed out representative CD3 positive T cells. e–g CD3 immunostaining showed that ATF4 inactivation specifically in oligodendrocytes did not significantly change the number of CD3 positive T cells in the molecular layer and internal granular layer of the cerebellum (CB) in EAE mice at PID21. Red fluorescence: CD3, blue fluorescence: DAPI. Arrows pointed out representative CD3-positive T cells. h, i, n CD11b immunostaining showed that ATF4 inactivation specifically in oligodendrocytes did not significantly change the number of CD11b-positive microglia/macrophages in the anterior horn of the spinal cord (SC) in EAE mice at PID21. Red fluorescence: CD11b, blue fluorescence: DAPI. Arrows pointed out representative CD11b-positive microglia/macrophages. j, k, n CD11b immunostaining showed that ATF4 inactivation specifically in oligodendrocytes did not significantly change the number of CD11b-positive microglia/macrophages in the layer V of the primary motor cortex (MC) in EAE mice at PID21. Red fluorescence: CD11b, blue fluorescence: DAPI. Arrows pointed out representative CD11b-positive microglia/macrophages. l–n CD11b immunostaining showed that ATF4 inactivation specifically in oligodendrocytes did not significantly change the number of CD11b-positive microglia/macrophages in the molecular layer and internal granular layer of the cerebellum (CB) in EAE mice at PID21. Red fluorescence: CD11b, blue fluorescence: DAPI. Arrows pointed out representative CD11b-positive microglia/macrophages. Scale bars: a–f, h–m, 50 μm. N = 4 animals. Error bars represent SD, n.s. not significant