Fig. 5
IFNβ1a effects on microglial cell activation by Aβ1-42. a Iba-1 immunohistochemistry in the hippocampus. b Count of Iba-1-positive cells and c quantitative Western blot analyses of Iba-1 protein levels. b The count of Iba-1-positive cells showed a significant increase of cell number in the hippocampus region of the Aβ1-42-treated group (n = 6) and treatment with IFNβ1a (n = 6) significantly counteract this effect in the Aβ1-42+ IFNβ1a group (n = 6) as compared to sham group but not to the control group. c Similarly, quantitative Western blot analyses of Iba-1 protein levels showed that in the Aβ1-42 group, Iba-1 protein levels were significantly increased and treatment with IFNβ1a significantly counteract this effect in the Aβ1-42 + IFNβ1a group. Scale: 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001