Fig. 4

Effects of PGF and VEGF inhibition on mononuclear phagocytes in RPE/choroidal flat mounts. a Representative images of Iba1-stained microglia/macrophages in single laser spots of RPE/choroidal flat mounts 3 and 7 days after laser coagulation. Scale bar: 100 μm. b Quantification of mononuclear phagocytes per laser spot in retinal flat mounts 3 days after laser-induced damage (n = 20 laser spots for IgG, n = 10 laser spots for aVEGF, n = 12 laser spots for aPGF, n = 13 laser spots for aVEGF/aPGF, n = 27 laser spots for aflibercept). c Quantification of mononuclear phagocytes per laser spot in RPE/choroidal flat mounts 7 days after laser-induced damage (n = 11 laser spots for IgG, n = 10 laser spots for aVEGF, n = 10 laser spots for aPGF, n = 15 laser spots for aVEGF/aPGF, n = 30 laser spots for aflibercept). d Quantification of Iba1 signals 3 days after laser coagulation in RPE/choroidal flat mounts by counting the mean of colored pixels per image (n = 20 laser spots for IgG, n = 10 laser spots for aVEGF, n = 12 laser spots for aPGF, n = 13 laser spots for aVEGF/aPGF, n = 27 laser spots for aflibercept). e Quantification of Iba1 signals 7 days after laser coagulation in RPE/choroidal flat mounts by counting the mean of colored pixels per image (n = 11 laser spots for IgG, n = 10 laser spots for aVEGF, n = 10 laser spots for aPGF, n = 15 laser spots for aVEGF/aPGF, n = 30 laser spots for aflibercept). f Interleukin 6 (IL-6) levels in RPE/choroidal flat mounts 6 h after laser damage quantified by ELISA (n = 8 flat mounts per group). Naive (not lasered) animals were used as controls. g Interleukin 1β (IL-1β) levels in RPE/choroidal flat mounts 6 h after laser damage quantified by ELISA (n = 8 flat mounts per group). h Tumor necrosis factor (TNF) levels in RPE/choroidal flat mounts 6 h after laser damage quantified by ELISA (n = 8 flat mounts per group). Data are shown as mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001)