Fig. 6

Effects of PGF and VEGF inhibition on their transcription and secretion in phagocytes. a Representative images of in situ hybridization (ISH) in single laser spots of RPE/choroidal flat mounts 7 days after laser damage. Probes bind specifically to mRNAs of PGF and VEGF. Scale bar: 100 μm. b Mean RNA expression of VEGF per laser spot in RPE/choroidal flat mounts 7 days after laser coagulation. Expression was determined by quantifying the mean colored pixels per area (n = 10 laser spots for IgG, n = 10 laser spots for aVEGF, n = 11 laser spots for aPGF, n = 10 laser spots for aVEGF/aPGF, n = 14 laser spots for aflibercept). c Mean RNA expression of PGF per laser spot in RPE/choroidal flat mounts 7 days after laser coagulation. Expression was determined by quantifying the mean colored pixels per area (n = 10 laser spots for IgG, n = 10 laser spots for aVEGF, n = 11 laser spots for aPGF, n = 10 laser spots for aVEGF/aPGF, n = 14 laser spots for aflibercept). d Cytokine levels of VEGF in RPE/choroidal flat mounts 7 days after laser damage measured by ELISA (n = 6 flat mounts per group). e Cytokine levels of PGF in RPE/choroidal flat mounts 7 days after laser damage measured by ELISA (n = 6 flat mounts per group). Data are shown as mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001)