Fig. 3

Sema4A treatment causes loss of mature myelinating oligodendrocytes. The brains treated with PBS or Sema4A were sliced and stained for Olig2, a pan-oligodendrocyte marker, or CC1, a mature myelinating oligodendrocyte marker. a PBS injection causes no loss in total oligodendrocyte numbers. b Sema4A injection causes a decrease in total oligodendrocytes. c 50% oligodendrocyte loss is observed after Sema4A treatment. d PBS injection causes no loss in mature myelinating oligodendrocytes. e Sema4A injection causes a decrease of mature myelinating oligodendrocytes by 47% (f). PBS and Sema4A groups each had n = 5. Unpaired t tests were used to compare PBS and Sema4A groups; *p < 0.05