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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: The agonistic TSPO ligand XBD173 attenuates the glial response thereby protecting inner retinal neurons in a murine model of retinal ischemia

Fig. 4

Müller glial reactivity in the postischemic retina. a Top, retinal slices from control and 7 days post-surgery (dps) eyes were labeled for TSPO and counterstained for the Müller cell marker glutamine synthetase (GLUL). Colabeling of TSPO and GLUL in Müller cell processes and end feet are pointed out by blue arrowheads. Middle, immunolabeling for the microglia marker AIF1 and glial fibrillary acidic protein (GFAP), a marker for Müller cell gliosis. Bottom, immunoreactivity for DBI partially colocalizes to that of GLUL. White arrowheads point at putative microglia. Expression of Gfap (b), Dbi (c), and Glul (d) was tested by qPCR from MACS-enriched Müller cells of control (c), 3, 7, and 14 dps. bd Bars represent mean values ± SEM (n = 3–6 mice of each treatment group and time point). */°/•P < 0.05; °°P < 0.01; °°°/•••P < 0.001. The color of the circle indicates the treatment group. e Retinal flatmounts labeled for GLUL and focused on the ganglion cell layer (GCL) demonstrating reduced expression of GLUL at 3 dps which are more pronounced in postischemic retina of vehicle controls. a, e Scale bars, 50 μm

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