Fig. 5

Müller cells maintain part of their homeostatic functions in the XBD173-treated postischemic retina. a At 7 dps, Müller cells from wild-type mice display a slight increase of the mean soma area determined in vital retinal slices on basis of labeling with Mitotracker Orange, which was not observed in XBD173-treated mice. b Left, the ability of Müller cells to maintain their volume constant was tested by challenging them with a hypoosmotic solution for 4 min. An efficient Müller cell volume regulation was significantly better preserved in XBD173-treated postischemic retinae 7 dps, while Müller cells from control eyes did not swell at all. Right, representative images from Müller cell somata loaded with Mitotracker Orange before and after 4 min of hypoosmotic stress. a, b Bars represent mean values ± SEM from 8 to 20 cells out of 2–3 animals. **P < 0.01. Scale bars, 10 μm