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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: The agonistic TSPO ligand XBD173 attenuates the glial response thereby protecting inner retinal neurons in a murine model of retinal ischemia

Fig. 6

Expression of genes involved in neurosteroid biosynthesis in different retinal cell populations. a Scheme of neurosteroid synthesis from cholesterol where one rate-limiting step is its import into mitochondria. b Bar diagrams in (1) reflect the expression of the only cytochrome P450 family genes (Cyp2d11, Cy2d26, Cyp20a1, Cyp27a1) found to be expressed at protein level determined by quantitative LC-MS/MS mass spectrometry in the distinct retinal cell types. (2–4) Expression of enzymes for further conversion of pregnenolone to progesterone and other neuroactive steroids was low and only detectable at transcript level by RNA sequencing. (2) The highest expression of 3β-hydroxysteroid dehydrogenase (3β-HSD) type VII (Hsd3b7) were detected in Müller cells. (3) Two subtypes (Srd5a1, Srd5a3) of steroid 5α-reductase (5α-R) are ubiquitously expressed in all retinal cell types. (4) Akr1c13 belonging to aldo-keto reductase family 1 gene family (like 3α-hydroxysteroid dehydrogenase (3α-HSD)) is expressed at low levels and rather specifically in Müller glia. Fpkm, fragments per kilobase million. (5) High protein levels of the progesterone receptor membrane component 1 (PGRMC1) were detected in Müller glia and retinal neurons. Mc, Müller cells; n, neurons; mg, microglia; vc, vascular cells. Hsd3b7, 3β- and steroid δ-isomerase 7; Srd5a1, steroid 5 alpha-reductase 1; Srd5a3, steroid 5 alpha-reductase 3; Akr1c13, aldo-keto reductase family 1, member C13

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