Fig. 6
MANF protects against Aβ1–42 toxicity via inhibiting ER stress. a Effect of MANF overexpression on the levels of BiP, ATF6, phospho-IRE1, XBP1s, phospho-eIF2α, ATF4, CHOP, and cleaved caspase-3 in Aβ1–42-treated cells. N2a cells were transfected with pcDNA3.1-MANF-Flag plasmid and its empty vector before Aβ1–42 (10 μM) treatment for 24 h or TM (2.5 μg/ml) treatment for 12 h and processed for WB. b Effect of MANF knockdown on the levels of BiP, ATF6, phospho-IRE1, XBP1s, phospho-eIF2α, ATF4, CHOP, and cleaved caspase-3 in Aβ1–42-treated cells. N2a cells were transfected with NC-siRNA or MANF-siRNA before Aβ1–42 (10 μM) treatment for 24 h or TM (2.5 μg/ml) treatment for 12 h, respectively, then processed for WB. c Quantitation of proteins normalized to α-tubulin levels in a. d Quantitation of proteins normalized to α-tubulin levels in b. All the quantitative data were presented as mean ± SD of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.01. TM tunicamycin, C-caspase-3 cleaved caspase-3