Fig. 3
Microglia treated with LPS & FeSO4 increased primary neuronal toxicity in co-culture. a Immunocytochemistry of NeuN-positive primary neurons after 24-h microglial co-culture revealed reduced number of neurons and neurite network when exposed to LPS or LPS & FeSO4. Although FeSO4 alone did not result in a significant reduction, a lower number of neurons and decreased density of neurite network was noted. The addition of DFO ameliorated the toxic effect of microglia treated with LPS alone and LPS & FeSO4. b Quantification of NeuN-positive cells illustrated the toxic effect of LPS alone and in combination with FeSO4. Representative sample of 1 trial of 4 shown. Graph represents a sample size of 4. *p < 0.05; **p < 0.01, one-way ANOVA with Tukey’s post-hoc test. Bars represent mean ± SEM. Size bars represent 50 μm