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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Iron accentuated reactive oxygen species release by NADPH oxidase in activated microglia contributes to oxidative stress in vitro

Fig. 4

Iron accentuated ROS generation is significantly attenuated by NOX2 and NOX4 inhibition among activated microglia. a FeSO4 treatment in primary microglia led to a combinatorial effect with LPS that was diminished completely by GSK2795039 and GKT137831. b FeSO4 treatment among microglia induced a significant increase in ROS. LPS & DMSO did not increase ROS levels alone; however, the addition of FeSO4 to the group significantly increased ROS production. NOX2 inhibition with GSK2795039 significantly reduced ROS production to baseline. No significant differences in LDH or cell counts were detected between treatment groups within the GSK2795039 experiments. c Robust ROS production among microglia treated with FeSO4, LPS alone, or in combination. The addition of NOX4 inhibition of GKT137831 significantly reduced the presence of ROS. No significant differences in LDH or cell counts were detected between treatment groups within the GKT137831 experiments. In all graphs, symbols representing significance were assigned according to comparisons: DMSO group (*); FeSO4 & DMSO (#); and FeSO4 & LPS & DMSO ($). **p < 0.01, ***p < 0.001, ****p < 0.0001, ###p < 0.001, and $$$$p < 0.0001. All groups were compared using one-way ANOVA with Tukey’s post-hoc test. All graphs represent n = 6. Bars represent mean ± SEM

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