Fig. 3

Effect of macrophage deprivation on LPS-induced IL-1β immunoreactivity in the SFO. Cryosections were immunostained with antibodies against the outer basement membrane marker laminin-111, the myeloid lineage cell marker Iba1, and IL-1β. a Localization of peripherally injected control liposomes in the SFO. DiI-labeled control liposomes were found in the SFO perivascular space and co-localized with Iba1 immunoreactivity. b IL-1β immunoreactivity was found in the perivascular space (arrows) of the SFO following LPS injection in liposome (−) mice and control empty liposome-injected mice. A decreased number of Iba1⁺ cells and attenuated IL-1β immunoreactivity were seen in the perivascular space of the SFO following LPS injection in clodronate liposome-injected mice. c Quantitative analysis revealing the effects of clodronate liposome injection on the number of Iba1⁺ cells in the SFO of adult mice. N = 4. *P < 0.05, determined by one-way ANOVA with Scheffé’s post hoc test. d Quantitative analysis revealing the effects of clodronate liposome injection on the number of IL-1β⁺ Iba1⁺ cells in the perivascular space of the adult mice SFO. N = 4. **P < 0.01, determined by one-way ANOVA with Scheffé’s post hoc test. clod, clodronate liposomes; cont, control empty liposomes; Laminin, laminin-111; p, parenchymal area; v, vasculature. Scale bars are 10 μm