Fig. 4
Tnf and C5ar1 transcripts are reduced in lumbar spinal cord of anti-HMGB1 antibody-treated SOD1G93A mice. SOD1G93A mice were intraperitoneally injected weekly with the anti-HMGB1 antibody at 35 days of age (100 μg). Pro-inflammatory cytokines (TNFα and IL-1β) and major innate immune receptors (RAGE, C5aR1 and TLR4) in vehicle and anti-HMGB1-treated SOD1G93A mice was investigated at mid-symptomatic stage of disease (133 days) using quantitative PCR. a Show anti-HMGB1 treatment reduces pro-inflammatory cytokine Tnf in the spinal cord of SOD1G93A mice (n = 6, **p < 0.01, Student’s t test), while no change in Il1β was evident between isotype control and anti-HMGB1-treated SOD1G93A mice (b; n = 6, p > 0.05, Student’s t test). Anti-HMGB1 treatment showed slight reduction in C5ar1 mRNA transcript levels while no change was observed for Ager and Tlr4 (c–e; n = 6, *p < 0.05, Student’s t test). Data are presented as mean ± SEM