Fig. 5From: Therapeutic blockade of HMGB1 reduces early motor deficits, but not survival in the SOD1G93A mouse model of amyotrophic lateral sclerosisMonocyte and macrophage markers in tibialis anterior and gastrocnemius muscle are not altered between isotype control and anti-HMGB1 antibody-treated SOD1G93A mice. SOD1G93A mice were intraperitoneally injected weekly with the anti-HMGB1 antibody at 35 days of age (100 μg). Monocytes and macrophage markers in tibialis anterior (TA) and gastrocnemius (GN) muscle of vehicle and anti-HMGB1-treated SOD1G93A mice was investigated at mid-symptomatic stage (133 days) using quantitative PCR. a–c Shows anti-HMGB1 treatment had no effect on macrophage (Itgam, Cd68 and Aif1) mRNA transcript levels in both TA and GN muscles (n = 6, p > 0.05, Student’s t test). d Shows a reduction in monocyte (Ly6c) mRNA transcript levels in TA muscle of anti-HMGB1-treated SOD1G93A mice, while no change was evident in GN muscle when compared to isotype control-treated SOD1G93A mice (n = 6, *p < 0.05, Student’s t test). Data are presented as mean ± SEMBack to article page