Table 2 Primary antibodies
From: Astrocytic ceramide as possible indicator of neuroinflammation
Primary antibody | Clone | Dilution | Species raised in | Source |
|---|---|---|---|---|
Ceramide | MID 15B4 | 1:100 | Mouse | Alexis, Lausen, Switzerland [33] |
ASM | Â | 1:200 | Rabbit | Santa Cruz, California, USA [33] |
CerS5 | Â | 1:500 | Rabbit | Abcam, Cambridge, UK |
CerS2 | Â | 1:500 | Rabbit | Abcam, Cambridge, UK |
GFAP | Â | 1:2000 | Rabbit | DAKO, Glostrup, Denmark |
LN3 | Â | 1:500 | Mouse | VUmc, Amsterdam, The Netherlands |
GFAP-Cy3 | Â | 1:300 | Mouse | Sigma, MO, USA |
LN3-Alexa 488 | Â | 1:400 | Mouse | Vumc, Amsterdam, The Netherlands |