Fig. 8

ALX suppresses DC maturation in EAE mice. On day 15 post-induction, five mice from each group were sacrificed. The total number of splenic mononuclear cells and DCs were calculated. Splenic mononuclear cells were stained with anti-CD80, anti-CD86, and anti-MHC II. The levels of CD80, CD86, and MHC II expression on DCs indicate the degrees of DC maturation. The levels of IL12, IL-23, and IL-6 in cultured cell supernatants of individual mice were determined by ELISA. Data are representative images or expressed as the mean ± SEM of each group (n = 5) from three separate experiments. a Total number of splenic mononuclear cells and DCs. b Flow cytometry analysis of splenic DC maturation. The percentage of splenic CD80+CD11c+, CD86+CD11c+, and MHC II+CD11c+ cells in individual mice from different group. The MFI of CD80, CD86, and MHC II on DCs. c The expression levels of IL-12, IL-23, and IL-6 in supernatants of cultured splenocytes.^※p < .05, *p < .01, ^#p < 0.001, NS means no statistical difference