Fig. 1
IL-24 mRNA expression is induced in the CNS of mice following in vivo bacterial infection. Wild type C57BL/6J mice were uninfected (control, animal numbers 1 and 2) or infected by direct intracranial administration with Streptococcus pneumoniae (1 × 107 bacteria, animal numbers 3–6). At 72 h following infection, whole brain tissue was collected and expression of mRNA encoding for IL-24 was determined by semi-quantitative RT-PCR, and C57BL/6J mouse whole thymus tissue was used as a positive control (+). Relative IL-24 mRNA expression was determined by densitometric analysis and normalized to the level of the housekeeping gene GAPDH, and all data points and the mean are shown