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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Murine astrocytes produce IL-24 and are susceptible to the immunosuppressive effects of this cytokine

Fig. 4

Primary murine glia constitutively express the IL-22Rα subunit of the Type II receptor for IL-24. a Murine astrocytes were unstimulated or challenged with LPS (1 or 5 ng/ml) for 6 or 24 h, and levels of mRNA encoding IL-22Rα and GAPDH were determined by semi-quantitative RT-PCR. b Astrocytes were uninfected or infected with N. meningitidis (Nm), S. aureus (Sa), or S. pneumoniae (Sp) at MOI of 1, 10, or 50 bacteria to glia for 24 h prior to immunoblot analysis for IL-22Rα expression. Expression of β-actin is shown as a loading control, and relative IL-22Rα expression was determined by densitometric analysis and normalized to untreated cells. Data is expressed as the mean ± the SEM of 3 independent experiments, and an asterisk indicates a statistically significant difference from unchallenged cells (p < 0.05). c Murine microglia were uninfected or infected with N. meningitidis (Nm), S. aureus (Sa), or S. pneumoniae (Sp) at MOI of 1, 10, or 50 bacteria to glia for 24 h prior to immunoblot analysis for IL-22Rα expression. Expression of β-actin is shown as a loading control and relative IL-22Rα expression was determined by densitometric analysis and normalized to untreated cells. Data is expressed as the mean ± the SEM of three independent experiments

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