Skip to main content
Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Intrathecal triamcinolone acetonide exerts anti-inflammatory effects on Lewis rat experimental autoimmune neuritis and direct anti-oxidative effects on Schwann cells

Fig. 6

In vitro analyses of Schwann cells culture. a Immunohistochemical staining of Schwann cell culture with SOX10 and DAPI (nuclear staining). b, c After incubation of Schwann cells with 10 μg/ml triamcinolone (TRIAM) for 24 h, we detected a significant reduction in the relative gene expression of the pro-inflammatory TLR-4 as well as an increase in the anti-inflammatory cytokine IL-1Ra by RT-PCR. Mean values and SD are depicted. d Incubation of Schwann cells for 24 h with 0.03 mM H2O2. Co-incubation with 10 μg/ml triamcinolone resulted in increased cell viability in propidium iodide (PI) staining. Mean values and SD are depicted. e Incubation of Schwann cells with 100 U/ml interferon-γ. After co-incubation with triamcinolone, MHC II expression in flow cytometric analyses was significantly increased. Mean values and SD are depicted. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, and **** p ≤ 0.0001

Back to article page