Fig. 3
From: Loss of NF-κB p50 function synergistically augments microglial priming in the middle-aged brain
Loss of NF-κB p50 function and aging synergistically interact to augment midbrain IL-1β in middle-aged mice. Young adult (1.5–3.0 month old) and middle-aged (16.0–18.0 month old) NF-κB p50+/+ and NF-κB p50−/− mice were treated with a saline or b LPS (5 mg/kg, IP), and brain tissue was collected at 3 h post-injection. IL-1β expression in the brain was evaluated by quantitative RT-PCR. Values are normalized to GAPDH using the 2−ΔΔCT method and are reported as mean percent of NF-κB p50+/+ young adult saline control ± SEM. An asterisk indicates significant difference (P < 0.05) from the 1.5–3.0-month old group and a dagger indicates a significant difference between mouse strains. n = 3