Fig. 2

Orexin A significantly decreases the histopathological score of EAE mice and CD4⁺ T cell recruitment in the CNS. On day 21 post-immunization, spinal cord sections from naïve, PBS, and OxA₃₀₀ IP mice were prepared. a:Spinal cord tissues were stained with hematoxylin/eosin (to distinguish cell infiltration) and luxol fast blue (to detect myelin). b Spinal cord sections were labeled with DAPI and Alexa 488 anti-CD4. All images were taken at × 20 magnification. Two or three representative areas of spinal cord are shown per experimental group. Arrows show inflammatory and demyelinating areas. Graphs show the mean histological scores and the quantification of infiltrated CD4⁺ cells per mm² of each group (n = 7/group). *P < 0.05 (Mann-Whitney test, compared to PBS group)