Fig. 3

Orexin A significantly reduces inflammatory responses in the CNS. The panel shows the mRNA expression of chemokines (i.e., MCP-1 or CCL2 and IP-10 or CXCL10), cytokines (i.e., TNF-α, IL-10, IFN-γ (Th1), IL-17 (Th17), IL-4 (Th2), and TGF-β) and the transcription factor, Foxp3 (Treg-specific) determined by real time RT-PCR in naïve animals and EAE mice (i.e., 15 and 21 days after EAE induction in PBS- and OxA-treated mice, n = 6/group and n = 7/group respectively). *P < 0.05 (Mann-Whitney test, compared to PBS group)