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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Systemic administration of orexin A ameliorates established experimental autoimmune encephalomyelitis by diminishing neuroinflammation

Fig. 5

Orexin A receptors are widely expressed in the immune and nervous systems. The expression profile of the receptors OX1R and OX2R was determined in naĆÆve animals by PCR. RNA was prepared and reversed transcribed from a immune (cervical/axillary lymph nodes (cLN and aLN, respectively), thymus, spleen) and CNS tissues (brain and spinal cord) and b different immune cell populations such as T CD4+, T CD8+, and CD11b+ splenocytes that were sorted by flow cytometry using the FACS Aria-IIIu (BD Bioscience). The results were assayed by electrophoresis

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