Fig. 1

A flow diagram representing the manual gating strategy used to isolate major immune cell populations from mass cytometry output. Firstly, quality control gates were performed to remove normalisation beads, isolate singlets and gate CD45⁺ leukocytes. Next, mononuclear leukocytes (CD66⁻CD61b⁻CD235⁻) were isolated by gating out platelets, granulocytes and erythrocytes. Major lymphocyte and myeloid populations were then isolated using standard phenotypic markers. For example, CD19⁺ for B lymphocytes, CD3⁺ for T lymphocytes, CD56⁺ for NK cells and HLA-DR⁺ for myeloid cells. Finally, major cell populations were gated further into known subsets