Fig. 3

Increased numbers of central memory CD4⁺ T lymphocytes with increased pro-inflammatory activation are found in the blood of CRPS participants compared to healthy controls. a A bar graph showing the number of cells in FlowSoM clusters representing the four major CD4⁺ T lymphocyte populations; naive (Tn, CD3⁺CD4⁺CD45RA⁺CCR7⁺), effector (Teff, CD3⁺CD4⁺CD45RA⁺CCR7⁻), effector memory (Tem, CD3⁺CD4⁺CD45RO⁺CCR7⁻), central memory (Tcm, CD3⁺CD4⁺CD45RO⁺CCR7⁺). b A bar graph showing the number of cells in FlowSoM clusters representing central memory Th1 lymphocytes (CD3⁺CD4⁺CD45RO⁺CCR7⁺T-bet⁺) and Tregs (CD3⁺CD4⁺CD45RO⁺CCR7⁺CD127^loCD25⁺FoxP3⁺). Intracellular signalling and functional marker expression levels in FlowSoM clusters representing c Th1 lymphocytes and d Tregs. N = 14 CRPS and N = 14 controls. All data are presented as group means (± S.E.M.). * represents P < 0.05 and ** represents P < 0.01 in an unpaired Student’s T Test, and ^# represents P < 0.01 in a Mann-Whitney U Test