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Table 2 A table showing the mass cytometry panel used to immunophenotype the blood of CRPS and healthy control participants. The columns represent the rare-earth metal isotype used for conjugation, species reactivity, target antigen, clone, manufacturer and staining concentration. The antibody from Fluidigm was purchased metal labelled, and antibodies from all other manufacturers were purchased, metal-conjugated and validated in-house by the Ramaciotti Facility for Human Systems Biology

From: Expansion and activation of distinct central memory T lymphocyte subsets in complex regional pain syndrome

Metal isotype

Reactivity

Antigen

Clone

Manufacturer

Staining conc. (μg/ml)

Staining step buffer

110Pd

Mouse, Human

CD45

30-F11

BD

6

FACS

115In

Human

CD8

RPA-T8

Biolegend

4

FACS

141Pr

Human

CD235ab

HIR2

Biolegend

1.6

FACS

142Nd

Human

CD19

HIB19

BD

1

FACS

143Nd

Human

CD56

REA196

Miltenyi Biotec

0.5

FACS

144Nd

Human

TCR γδ

B1

BD

2

FACS

145Nd

Human

CD4

RPA-T4

BD

0.5

FACS

146Nd

Human

DAP12

406288

R&D systems

1

Perm. buffer

147Sm

Human, Mouse

TREM-2

237920

R&D systems

8

FACS

148Nd

Human

CD16

3G8

BD

1.5

FACS

149Sm

Human

CD25

2A3

BD

1

FACS

150Nd

Mouse, Human

pSTAT5 [pY694]

47

BD

1.5

Methanol

151Eu

Human

CD123 (IL-3R)

6H6

Biolegend

1

FACS

152Gd

Human

CD66b

80H3

Fluidigm

3 (μl/ml)

FACS

153Eu

Mouse, Human

pSTAT1 [pY694]

14/P-Stat1

BD

2

Methanol

154Gd

Mouse, Human

pAKT [T308]

J1.233.371

BD

4

Methanol

155Gd

Human

CD354 (TREM-1)

TREM-37

Biolegend

2

FACS

156Gd

Human

pp38 MAPK [T180/Y182]

30/p38 MAPK

BD

4

Methanol

158Gd

Human, Mouse

pSTAT3 [Y705]

4/P-STAT3

BD

20

Methanol

159Tb

Human

CD197 (CCR7)

G043H7

Biolegend

8

FACS

160Gd

Human

CD14

M5E2

Biolegend

6

FACS

161Dy

Human

CD141 (BDCA3)

AD5-14H12

Miltenyi Biotec

8

FACS

162Er

Human

Foxp3

PCH101

eBioscience

3

Perm. buffer

163Dy

Human

CD1c

L161

Biolegend

1

FACS

164Er

Human

CD45RO

UCHL1

Biolegend

6

FACS

165Ho

Human

CD61 (Integrin β 3)

VI-PL2

Biolegend

1

FACS

166Er

Human

pp65 (NFκB) [S529]

K10-895.12.50

BD

4

Methanol

167Er

Human

CD11c

Bu15

Biolegend

1

FACS

168Er

Human

pERK 1/2 [Y202/204]

4B11B69

Biolegend

2

Methanol

169Tm

Human

CD45RA

HI100

Biolegend

2

FACS

170Er

Human

CD3

UCHT1

BD

2

FACS

171Yb

Human

Arginase-1

14D2C43

Biolegend

4

Perm. buffer

172Yb

Human

CD130

AM64

BD

8

FACS

173Yb

Human, Mouse

pMAPKAPK-2 [T334]

P24-694

BD

4

Methanol

174Yb

Human

HLA-DR

G46-6

BD

2

FACS

175Lu

PE

PE

PE001

Biolegend

4

FACS

176Lu

Human

CD127

A019D5

Biolegend

2

FACS

209Bi

Human, Mouse

T-bet

4B10

BD

1

Perm. buffer

n/a

Human

pPLC γ 2 [Y759]-PE

K86-689.37

BD

200 (μl/ml)

Methanol