Expansion and activation of distinct central memory T lymphocyte subsets in complex regional pain syndrome

Table 4 Significant increases in major T lymphocyte populations in the blood of CRPS participants. A table showing the numbers of major immune cell populations in the blood of CRPS and healthy controls based on manual gating analysis of mass cytometry output. N = 14 CRPS and N = 14 controls. All data are presented as group means (± S.E.M.). * represents P < 0.05 in unpaired Student’s T test, ^# represents P < 0.05 in Mann-Whitney U test

Cell population	Control (cells/ml)	± S.E.M.	CRPS (cells/ml)	± S.E.M.
CD3⁺TCRγδ⁻ T cells	446,868	34,128	657,776*	92,487
CD3⁺TCRγδ⁻CD4⁺ T cells	263,243	20,573	396,510	63,516
CD4⁺CD45RA⁺ Naive T cells	103,023	13,699	154,529	26,565
CD4⁺CD45RO⁺ Memory T cells	144,787	11,592	208,773	39,029
CD4⁺CD45RO⁺T-bet⁺ Memory Th1 cells	51,318	6575	101,242	23,548
CD4⁺CD127^loCD25⁺ Tregs	14,782	2127	32,343	9936
CD4⁺CD127^loCD25⁺CD45RA⁺ Naive Tregs	4255	909	8190	2653
CD4⁺CD127^loCD25⁺CD45RO⁺ Memory Tregs	10,421	1365	23,934	7461
CD4⁺CD127^loCD25⁺FoxP3⁺ Tregs	5573	485	11,925*	2736
CD3⁺TCRαβ ⁺ CD8⁺ T cells	183,550	18,908	261,338	40,889
CD8⁺CD45RA⁺ Naive T cells	108,545	16,263	142,125	23,135
CD8⁺CD45RO⁺ Memory T cells	74,962	7634	118,769	28,520
CD3⁺TCRαβ ⁺CD56⁺ NKT cells	10,092	1296	20,082	7371
CD3⁺TCRγδ ⁺ T cells	8384	1465	15,172	4273
CD19⁺ B cells	62,633	10,241	93,386	19,075
CD56⁺CD19⁻CD3⁻ NK cells	64,508	8369	103,364	19,520
HLA-DR⁺CD19⁻CD3⁻CD56⁻ Myeloid cells	199,230	28,780	260,322	33,995
HLA-DR⁺CD14⁺ Classical monocytes	158,330	24,822	207,083	29,342
HLA-DR⁺CD14⁺CD16⁺ Inflammatory monocytes	23,262	2984	28,884	3968
HLA-DR⁺CD14⁻CD11c⁺CD1c⁺CD141⁻ mDC	3101	433	1738^#	344
HLA-DR⁺CD14⁻CD11c⁺CD141c⁺CD1c⁻ mDC	1030	196	883	119
HLA-DR⁺CD14⁻CD123⁺ pDCs	2624	322	2968	727

ISSN: 1742-2094