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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Visualizing neuroinflammation with fluorescence and luminescent lanthanide-based in situ hybridization

Fig. 3

Luminescent lanthanide-based ISH staining in BV2 cells. a The detection of DAPI (blue; top panel) with epifluorescent microscopy and autofluorescence (red; bottom panel) with time-gated microscopy. b The detection of DAPI (blue; top panel) with epifluorescent microscopy and non-specific SA-Eu3+ binding (red; bottom panel) by time-gated microscopy. ce The detection of DAPI (blue; top panel) with epifluorescent microscopy and the detection of TLR4 mRNA (red) by luminescent lanthanide staining and time-gated microscopy at c 0 h, d 4 h, and e 24 h of LPS-induced inflammation. f TLR4 mRNA was significantly increased from 0 to 4 h following LPS stimulation and subsequently returned to near baseline levels of expression by 24 h. A filter with 343-nm excitation/441-nm emission was used for epifluorescent microscopy (DAPI filter); 340-nm excitation/615-nm emission was used for SA-BHHTEGST-Eu3+ time-gated microscopy (100x magnification; scale bars = 100 μm)

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