Fig. 1

The expression of IL-10 and IL-1β in microglia. a Microglia from mice in the control group (n = 6) and epileptic-seizure mice (n = 6) were incubated with or without LPS (1 μg/ml), and IL-10 mRNA was evaluated by RT-PCR. b Graphs show the ratios of IL-10/GAPDH. Data show mean ± SEM of four separate experiments. c, e The expression of IL-1β in LPS-stimulated microglia of mice from the control group (n = 6) and epileptic-seizure mice (n = 6). The level of IL-1β was assessed by RT-PCR. d, f Statistical results for the IL-1β/GAPDH ratios. Data represent mean ± SEM of four independent experiments. GAPDH was used for normalization. **P < 0.01. g, i Microglial cells from the control group of mice (g) (n = 6) and epileptic-seizure mice (i) (n = 6) were treated with or without LPS (1 μg/ml) in the presence or absence of exogenous IL-10, and the expression of IL-1β was measured by RT-PCR. h, j Quantitative data are mean ± SEM from three independent experiments. *P < 0.05