Fig. 2

IL-10 inhibits the production of IL-1β by microglial cells. a, b The secretion of IL-10 and IL-1β were assessed by ELISA in the supernatants of microglial cells from epileptic-seizure mice after LPS stimulation at different time points (n = 6). c Endogenous IL-10 suppressed IL-1β production by microglia during LPS stimulation (n = 6). Microglial cells were activated with LPS in the presence or absence of IL-10-neutralizing antibodies at different doses or of isotype-matched control antibodies for 12 h. The level of IL-1β was evaluated by ELISA. d Microglial cells were incubated with medium, LPS, increasing concentrations of IL-10, or isotype-matched control antibodies for 12 h. The level of IL-1β in supernatants was detected by ELISA (n = 6). All statistical results are shown as mean ± SEM from four independent experiments. *P < 0.05; **P < 0.01; ns not significant