Fig. 4

IL-10 reduces inflammasome activity and proIL-1β levels. a, b Microglia were incubated for 8 h with or without LPS. Amounts of procaspase-1 and caspase-1 were determined by Western blotting (n = 6). Expression levels of procaspase-1 and caspase-1 were normalized to amounts of GAPDH, and data represent mean ± SEM of four individual experiments; ns not significant. *P < 0.05. c–e IL-10 regulates IL-1β precursor levels. Microglial cells from epileptic seizure mice, STAT-1 and STAT-3 siRNA knockdown, respectively, were treated with LPS in presence or absence of exogenous IL-10. ProIL-1β levels were assessed in cell lysates (n = 6). **P < 0.01, ns not significant. f Microglial cells from STAT-3 siRNA knockdown mice were stimulated with LPS in the presence or absence of exogenous IL-10. Caspase-1 activation was assessed by Western blot (n = 6). A representative Western blot is shown from three independent experiments. ns not significant