Fig. 1

Six days of stress loading significantly prolonged pain behaviors. a Pressure pain threshold of the muscle. b Mechanical withdrawal threshold of the skin (von Frey test (VFT)). Note the significant decreases in pain threshold for 10 days (pressure pain threshold test) and skin pain for 8 days (VFT). NCS, no continuous stress; right, right leg or paw; left, left leg or paw. n = 5 (each group); **p < 0.01, ***p < 0.001 vs. NCS left; ††p < 0.01, †††p < 0.001 vs. NCS right. Two-way repeated measures ANOVA followed by the Holm-Sidak multiple comparisons test