Fig. 1

Inhibitory effects of GW9508 given intrathecally on mechanical allodynia (a, b) and thermal hyperalgesia (c, d) in neuropathic rats induced by spinal nerve ligation. Dose-response analyses of GW9508 on mechanical allodynia (b) and thermal hyperalgesia (d) were calculated using the thresholds measured 1 h after injection, best projected by the nonlinear least squares method. Effects of the selective GPR40 antagonist GW1100 (e) and GPR120 antagonist AH7614 (f) on GW9508-induced mechanical antiallodynia. Data are presented as means ± SEM (N = 6~7 per group). *P < 0.05 vs. the vehicle control group; analyzed by repeated measures two-way ANOVA followed by the post hoc Student-Newman-Keuls test