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Table 1 Dilution ratios of primary antibodies against GPR40, IL-10, β-endorphin, Iba-1, NeuN, and GFAP in immunocytochemistry and immunohistochemistry

From: Activation of GPR40 produces mechanical antiallodynia via the spinal glial interleukin-10/β-endorphin pathway

Primary antibody Manufacturer Dilution ratio
Cell flask Tissue section
GPR40 Santa Cruz Biotech, Santa Cruz, CA, USA 1:100 1:50
IL-10 R&D system, Minneapolis, MN, USA 1:200
β-Endorphin Phoenix Pharmaceuticals, Burlingame, CA, USA 1:100
Iba-1 Merck Millipore, Darmstadt, Germany 1:300 1:100
NeuN Merck Millipore, Darmstadt, Germany 1:100 1:60
GFAP Merck Millipore, Darmstadt, Germany 1:200 1:100