Table 1 Dilution ratios of primary antibodies against GPR40, IL-10, β-endorphin, Iba-1, NeuN, and GFAP in immunocytochemistry and immunohistochemistry
Primary antibody | Manufacturer | Dilution ratio | |
|---|---|---|---|
Cell flask | Tissue section | ||
GPR40 | Santa Cruz Biotech, Santa Cruz, CA, USA | 1:100 | 1:50 |
IL-10 | R&D system, Minneapolis, MN, USA | – | 1:200 |
β-Endorphin | Phoenix Pharmaceuticals, Burlingame, CA, USA | – | 1:100 |
Iba-1 | Merck Millipore, Darmstadt, Germany | 1:300 | 1:100 |
NeuN | Merck Millipore, Darmstadt, Germany | 1:100 | 1:60 |
GFAP | Merck Millipore, Darmstadt, Germany | 1:200 | 1:100 |