Fig. 2

Microglial numbers are not affected by GPR34 deficiency. a, b Contralateral dorsal horn in WT (a) and GPR34-deficient (b) mice at 1 day after L4 nerve injury. c–j Ipsilateral dorsal horn in WT (c, e, g, i) and GPR34-deficient (d, f, h, j) mice at 1 (c, d), 3 (e, f), 7 (g, h), and 14 (i, j) days after L4 nerve injury. Sections were stained by anti-Iba1 antibody (green) and anti-PKCγ antibody (red) for visualization of microglia and inner lamina II, respectively. Iba1-positive microglia within lamina I/IIo, identified by a dotted line, were counted. Scale bar = 200 μm. k Quantification of microglial numbers in lamina I/IIo of the ipsilateral and contralateral L4 dorsal horn in WT and GPR34-deficient mice after L4 nerve injury (n = 4 for each time point). Values are mean ± SEM. There was no significant difference between WT and GPR34-deficient mice at any time points (one-way ANOVA with post-hoc Tukey’s test)