Fig. 8From: Microglial NLRP3 inflammasome activation mediates IL-1β release and contributes to central sensitization in a recurrent nitroglycerin-induced migraine modelIL-1R was mainly expressed in the TNC neurons. Double immunofluorescence staining for IL-1R (green) with NeuN, a neuronal marker (red); GFAP, an astrocyte marker (red); or Iba-1, a microglial marker (red) in the mouse TNC after repeated nitroglycerin (NTG) administrations. a–e The results revealed the colocalization of IL-1R with NeuN in the TNC (white arrows). f–o The expression of IL-1R was not detected in GFAP- or Iba-1-positive cells. a–c, f–h and k–m × 10 objective lens; scale bars, 50 μm. d, i and n × 20 objective lens; scale bars, 20 μm. e, j, and o × 40 objective lens; scale bars, 20 μmBack to article page