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Table 2 Antibodies used in Western blot analysis, immunofluorescence staining and immunohistochemistry

From: Microglial NLRP3 inflammasome activation mediates IL-1β release and contributes to central sensitization in a recurrent nitroglycerin-induced migraine model

Antibody Manufacturer Catalogue number Host Dilution
For Western blot analysis
 NLRP3 Abcam, UK ab205680 Rat 1:1000
 IL-1β Santa Cruz, USA sc-515,598 Mouse 1:3000
 c-Fos Santa Cruz, USA sc-447 Mouse 1:1200
 p-ERK Cell Signaling, USA 5726 Mouse 1:1000
 CGRP Abcam, UK ab10062 Mouse 1:2000
 β-actin Proteintech, China 20,536–1-AP Rabbit 1:9000
 Anti-rabbit IgG (HRP) ZSGB-BIO, China ZB-2301 Goat 1:5000
 Anti-mouse IgG (HRP) ZSGB-BIO, China ZB-2305 Goat 1:5000
 Anti-rat IgG (HRP) ZSGB-BIO, China ZB-2307 Goat 1:5000
For immunofluorescence staining
 NLRP3 Biorbyt orb101128 Rabbit 1:400
 CGRP Santa Cruz, USA sc-57,053 Mouse 1:100
 IL-1β Santa Cruz, USA sc-515,598 Mouse 1:50
 IL-1R Abcam, UK ab124962 Rabbit 1:200
 Iba-1 Santa Cruz, USA sc-32,725 Mouse 1:200
 Iba-1 Wako, Japan 019–19,741 Rabbit 1:500
 GFAP Abcam, UK ab10062 Mouse 1:100
 NeuN Abcam, UK ab104224 Mouse 1:500
 Alexa Fluor 488 goat anti-rabbit IgG Beyotime, China A0423 Goat 1:400
 Alexa Fluor 488 goat anti-mouse IgG Beyotime, China A0428 Goat 1:400
 Cy3 goat anti-rabbit IgG Beyotime, China A0516 Goat 1:400
 Cy3 goat anti-mouse IgG Beyotime, China A0521 Goat 1:400
For immunohistochemistry
 c-Fos Abcam, USA ab208942 Mouse 1:1000