Antibody | Manufacturer | Catalogue number | Host | Dilution |
---|---|---|---|---|
For Western blot analysis | ||||
NLRP3 | Abcam, UK | ab205680 | Rat | 1:1000 |
IL-1β | Santa Cruz, USA | sc-515,598 | Mouse | 1:3000 |
c-Fos | Santa Cruz, USA | sc-447 | Mouse | 1:1200 |
p-ERK | Cell Signaling, USA | 5726 | Mouse | 1:1000 |
CGRP | Abcam, UK | ab10062 | Mouse | 1:2000 |
β-actin | Proteintech, China | 20,536–1-AP | Rabbit | 1:9000 |
Anti-rabbit IgG (HRP) | ZSGB-BIO, China | ZB-2301 | Goat | 1:5000 |
Anti-mouse IgG (HRP) | ZSGB-BIO, China | ZB-2305 | Goat | 1:5000 |
Anti-rat IgG (HRP) | ZSGB-BIO, China | ZB-2307 | Goat | 1:5000 |
For immunofluorescence staining | ||||
NLRP3 | Biorbyt | orb101128 | Rabbit | 1:400 |
CGRP | Santa Cruz, USA | sc-57,053 | Mouse | 1:100 |
IL-1β | Santa Cruz, USA | sc-515,598 | Mouse | 1:50 |
IL-1R | Abcam, UK | ab124962 | Rabbit | 1:200 |
Iba-1 | Santa Cruz, USA | sc-32,725 | Mouse | 1:200 |
Iba-1 | Wako, Japan | 019–19,741 | Rabbit | 1:500 |
GFAP | Abcam, UK | ab10062 | Mouse | 1:100 |
NeuN | Abcam, UK | ab104224 | Mouse | 1:500 |
Alexa Fluor 488 goat anti-rabbit IgG | Beyotime, China | A0423 | Goat | 1:400 |
Alexa Fluor 488 goat anti-mouse IgG | Beyotime, China | A0428 | Goat | 1:400 |
Cy3 goat anti-rabbit IgG | Beyotime, China | A0516 | Goat | 1:400 |
Cy3 goat anti-mouse IgG | Beyotime, China | A0521 | Goat | 1:400 |
For immunohistochemistry | ||||
c-Fos | Abcam, USA | ab208942 | Mouse | 1:1000 |