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Table 2 Antibodies used in Western blot analysis, immunofluorescence staining and immunohistochemistry

From: Microglial NLRP3 inflammasome activation mediates IL-1β release and contributes to central sensitization in a recurrent nitroglycerin-induced migraine model

Antibody

Manufacturer

Catalogue number

Host

Dilution

For Western blot analysis

 NLRP3

Abcam, UK

ab205680

Rat

1:1000

 IL-1β

Santa Cruz, USA

sc-515,598

Mouse

1:3000

 c-Fos

Santa Cruz, USA

sc-447

Mouse

1:1200

 p-ERK

Cell Signaling, USA

5726

Mouse

1:1000

 CGRP

Abcam, UK

ab10062

Mouse

1:2000

 β-actin

Proteintech, China

20,536–1-AP

Rabbit

1:9000

 Anti-rabbit IgG (HRP)

ZSGB-BIO, China

ZB-2301

Goat

1:5000

 Anti-mouse IgG (HRP)

ZSGB-BIO, China

ZB-2305

Goat

1:5000

 Anti-rat IgG (HRP)

ZSGB-BIO, China

ZB-2307

Goat

1:5000

For immunofluorescence staining

 NLRP3

Biorbyt

orb101128

Rabbit

1:400

 CGRP

Santa Cruz, USA

sc-57,053

Mouse

1:100

 IL-1β

Santa Cruz, USA

sc-515,598

Mouse

1:50

 IL-1R

Abcam, UK

ab124962

Rabbit

1:200

 Iba-1

Santa Cruz, USA

sc-32,725

Mouse

1:200

 Iba-1

Wako, Japan

019–19,741

Rabbit

1:500

 GFAP

Abcam, UK

ab10062

Mouse

1:100

 NeuN

Abcam, UK

ab104224

Mouse

1:500

 Alexa Fluor 488 goat anti-rabbit IgG

Beyotime, China

A0423

Goat

1:400

 Alexa Fluor 488 goat anti-mouse IgG

Beyotime, China

A0428

Goat

1:400

 Cy3 goat anti-rabbit IgG

Beyotime, China

A0516

Goat

1:400

 Cy3 goat anti-mouse IgG

Beyotime, China

A0521

Goat

1:400

For immunohistochemistry

 c-Fos

Abcam, USA

ab208942

Mouse

1:1000